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  • what is back pressure - Chromatography Forum
    It's called backpressure because it's established by the column lines and depends on the fluid viscosity, flow rate, temperature etc , not directly contralled by the pump setting, as the pressure is a consequence of the actual pump setting for the system resistance to flow
  • Column pressure estimation equation - Chromatography Forum
    Use a zero dead volume union in place of the column to obtain the system backpressure for each specific mixture (or solution), then add the column back in and measure again (subtract to obtain the column only value) Most CDS will even plot system pressure over time which is great for measuring the pressure change over a gradient method
  • Troubleshooting High Backpressure in HPLC System
    Recently, I’ve been experiencing unusually high backpressure during my runs, and I’m not sure what’s causing it The system had been running fine with a C18 column (4 6 x 150 mm, 5 µm particle size) and an acetonitrile water gradient (60:40), but now the backpressure is significantly higher than normal, even with the same mobile phase
  • What is back pressure ? Thank you - Chromatography Forum
    So backpressure is the pressure on the mobile phase required to overcome the resistance toward flow in the column and the plumbing?
  • Dionex IC 5000 EG backpressure - Chromatography Forum
    The backpressure generated at 1 mL min by the CR-TC is typically < 100 psi and by the RFIC EG degasser is typically < 250 psi I think your pressures are ok Are you having issues or just curious?
  • High backpressure from columns when running water only
    After a while the backpressure got excessively high, ~1200 psi from the guard column, and >3100 psi from the main column After reverting to MSA eluent, backpressures normalized rapidly (I first only inserted the guard column, and the backpressures normalized before the eluent could have reached the column)
  • Backpressure Calculation - Chromatography Forum
    Backpressure Calculation Johte Posts: 15 Joined: Thu Apr 09, 2015 11:52 am by Johte » Thu Aug 27, 2015
  • Increased backpressure after attachment of guard column
    I have recently purchased a REZEX ROA H+ column After attachment of the compatible guard column, backpressure of the system increased ( by ~150 psi) and the retention time got delayed (by ~ 3 mins) from the one mentioned in Certificate of Quality Assurance The column is brand new and we have just injected standards
  • waters E2695 normal backpressure without column
    The backpressure will increase greatly as the tubing ID decreases The Delta P you note is probably also a bit should be roughly less than 3 percent of the observed backpressure--and honestly most of the time more like less than 1 percent of the observed system backpressure 3% used to be Waters' recommendation as I recall
  • Increased backpressure after attachment of guard column
    1: Backpressure from System = 131 psi 2: Backpressure from Column = 537 -131 = 406 psi 4: Backpressure from Guard column = 334 - 131 = 203 psi Therefore the calculated pressure with column and guard column should be theoretically: 131 + 406 + 203 = 740 psi Your measured value 3) = 682 psi is about 9 % below of the calculated backpressure





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