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  • Paraquat exposure over generation affects lifespan and . . .
    We have investigated the effect of chronic mitochondrial stress by a low dose of paraquat (0 035 mM) over multiple generations in reproduction and lifespan of N2, isp-1(qm150), and cep-1(gk138) strains The fertility and lifespan data together indicate a trade-off between reproduction and longevity in chronic paraquat stress
  • Survival assays using Caenorhabditis elegans - PMC
    For example, we showed that low doses of paraquat (0 015 mM to 1 mM) extend the lifespan of C elegans, whereas high concentrations (4 mM to 64 mM) decrease lifespan (Hwang et al , 2014; Lee et al , 2010)
  • Paraquat exposure over generation affects lifespan and . . .
    It is important to understand the effects of acute and chronic stress caused by mitochondrial toxicants over multiple generations at the cellular and organism levels Using the model nematode C elegans, we show that acute and chronic exposure to paraquat affects reproduction, longevity, gene expression, and mitochondrial physiology
  • Low-dose N-acetyl cysteine prevents paraquat-induced . . .
    These findings explain why the 0 5 mM NAC dose was selected to mitigate PQ toxicity in our experiments Coincidentally, prolonged treatment with 5- or 15-mM NAC to young or aged C elegans dose-dependently reduced C elegans’ survival and lifespan (Gusarov et al , 2021)
  • Paraquat exposure over generation affects lifespan and . . .
    Chronic exposure to a low dose of paraquat (0 035 mM) over multiple generations in N2 causes a progressive decline of fertility, leading to complete loss of fertile embryo production by the third
  • Measuring Oxidative Stress in Caenorhabditis elegans:Paraquat . . .
    We make up a 1 M paraquat solution for the paraquat development assay and the chronic paraquat sensitivity assay This solution can be stored at 4 °C for many months In the paraquat development assay, we normally test multiple concentrations of paraquat: 0 1 mM, 0 2 mM, 0 3 mM and 0 4 mM
  • Photodynamic treatment increases the lifespan and oxidative . . .
    A low dose of the ROS-producing chemical paraquat reportedly increases the immunity and lifespan of Caenorhabditis elegans [7] The lifespans of C elegans and aging mice are extended by supplementation with ᴅ-glucosamine, an inhibitor of glycolysis that increases ROS production [ 8 ]





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