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  • Genome assembly using PacBio data - Galaxy Training Network
    Short summary generated by BUSCO indicates that Flye assembly contains: BUSCO analysis confirms that these two assemblies are of similar quality, with similar number of complete, fragmented and missing BUSCOs genes Conclusion This pipeline shows how to generate and evaluate a genome assembly from long reads PacBio data
  • Large genome assembly and polishing - Galaxy Training Network
    Assembly with Flye workflow What it does: Assembles long reads with the tool Flye Inputs: long reads (may be raw, or filtered, and or corrected); fastq gz format Outputs: Flye assembly fasta Fasta stats on assembly fasta Assembly graph image from Bandage Bar chart of contig sizes Quast reports of genome assembly Tools used: Flye Fasta
  • Assembly Large genome assembly and polishing Assembly with Flye . . .
    DNA sequence data has become an indispensable tool for Molecular Biology Evolutionary Biology Study in these fields now require a genome sequence to work from We call this a 'Reference Sequence ' We need to build a reference for each species We do this by Genome Assembly De novo Genome Assembly is the process of reconstructing the original DNA sequence from the fragment reads alone
  • Genome Assembly Quality Control
    In this tutorial, we will assess the assembly quality of 2 assemblies generated with Hifiasm and Flye using PacBio HiFi reads of a species of fungi, Saccharomyces cerevisiae INSC1019 and compare the results with the actual reference genome Saccharomyces cerevisiae S288C
  • Genome Assembly of MRSA from Oxford Nanopore MinION data (and . . .
    DNA sequence data has become an indispensable tool for Molecular Biology Evolutionary Biology Study in these fields now require a genome sequence to work from We call this a 'Reference Sequence ' We need to build a reference for each species We do this by Genome Assembly De novo Genome Assembly is the process of reconstructing the original DNA sequence from the fragment reads alone
  • Flye: de novo assembler for single molecule sequencing reads
    Collection of tutorials developed and maintained by the worldwide Galaxy community
  • Chloroplast genome assembly - Galaxy Training Network
    Assemble: Use Flye to assemble the nanopore reads, then get Fasta statistics Note: this may take several hours Polish assembly: Use Pilon to polish the assembly with short Illumina reads Note: Don’t forget to map these Illumina reads to the assembly first using bwa-mem, then use the resulting bam file as input to Pilon
  • Genome Assembly using PacBio data
    DNA sequence data has become an indispensable tool for Molecular Biology Evolutionary Biology Study in these fields now require a genome sequence to work from We call this a 'Reference Sequence ' We need to build a reference for each species We do this by Genome Assembly De novo Genome Assembly is the process of reconstructing the original DNA sequence from the fragment reads alone
  • Pathogen detection from (direct Nanopore) sequencing data using Galaxy . . .
    A microbiome is the community of microorganisms that can usually be found living together in any given habitat Microbiome research has grown substantially over the past decade in terms of the range of biomes sampled, identified taxa, and the volume of data derived from the samples
  • FAQs Chloroplast genome assembly Assembly - Galaxy Training Network
    The assembly process in Flye is heuristic, and the resulting assembly will not necessarily be exactly the same each time This may happen even if running the same data with the same version of Flye It can also happen with a different version of Flye To make things more complicated (stop reading now if you would like!)… the chloroplast genome has a structure that includes repeats (the





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